Gene Cloning Process Diagram

This diagram illustrates the fundamental steps of gene cloning, a powerful molecular biology technique. It shows how a specific gene of interest is isolated and inserted into a bacterial plasmid, creating a recombinant DNA molecule. The process begins with the enzymatic cutting of both the target DNA and the plasmid. The gene is then ligated into the plasmid, forming the recombinant construct. This modified plasmid is subsequently introduced into bacterial cells through transformation. A critical selection step, often involving antibiotics, ensures that only bacteria successfully carrying the recombinant plasmid (which typically includes an antibiotic resistance gene) survive and multiply, leading to the amplification of the desired gene.

gene cloning - plasmid - bacteria - dna - recombinant DNA - restriction enzyme - ligation - transformation - antibiotic resistance